Clsi Document M45 Pdf Updated Jun 2026
✅ listing each M45 organism, required media, atmosphere, incubation time, and which drugs have breakpoints. ✅ Stock rare supplement (e.g., lysed horse blood, ß-NAD) only if you see these organisms periodically. ✅ Train staff to recognize when to escalate to M45 testing – often prompted by failed identification or no commercial AST results. ✅ Document validation if you adapt M45 methods to automated systems (FDA requires separate clearance; research use only unless validated).
to input the specific M45 interpretive criteria for disk diffusion, particularly if not using standard M100 breakpoints. Validation: clsi document m45 pdf
No. M100 covers routine organisms ( E. coli , S. aureus , P. aeruginosa ). M45 covers special organisms. However, M100 often refers to M45 for testing methods of fastidious bacteria. ✅ listing each M45 organism, required media, atmosphere,
: Listeria monocytogenes , Corynebacterium species, Bacillus species (excluding B. anthracis ), and Erysipelothrix rhusiopathiae . ✅ Document validation if you adapt M45 methods
The document describes traditional broth microdilution and agar disk diffusion methods, including standardized procedures for performance, drug selection, and quality control (QC).
The CLSI M45 standard applies to veterinary clinical laboratories that perform antimicrobial susceptibility testing on bacteria isolated from animals. The standard covers the testing of aerobic and anaerobic bacteria, including those that are commonly isolated from animals.
